CARD R-BASE



Mouse strain


Strain information

CARD ID 3629
Type of strain Transgenic., Targeted mutant.
Strain name BALB/c-Jak3tm1Card Rag2tm1Card Tg(mouseSIRPα promoter-humanSIRPα)-HGNC:9662
Internal Code BRJ-SIRPαTg
Submitter Seiji Okada
Submitter affiliation or code Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection, Kumamoto University
Stock Type
Material Transfer Conditions Consent to us
Production method in-house breeding
Origin (In-house) Organization Kumamoto University
Organization code
Developer Seiji Okada
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol SIRPα
Gene name signal regulatory protein alpha [ Homo sapiens (human)
Allele symbol
Allele name
MGI MGI:108563,
Chromosome
Gene classification Gene to express(transgenic)
Method MicroInjection BAC Tg
OMIM
Gene symbol Jak3
Gene name Janus kinase 3
Allele symbol Jak3tm1card
Allele name Janus kinase 3; targeted mutation 1, Center for Animal Resources & Development,
MGI MGI:99928,
Chromosome
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM
Gene symbol Rag2
Gene name recombination activating gene 2
Allele symbol Rag2tm1card
Allele name recombination activating gene 2; targeted mutation 1, Center for Animal Resources & Development
MGI MGI:97849,
Chromosome
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
hSIRPaBIF6 GAGGACAAGTTGGAAAAGATGGT
hSIRPaBIR6 GGAGGTCTCATTACTGCTTC
PCR Primer 2
Neo-3 CCAACGCTATGTCCTGATAGCGGT
RG2-2 TTAATTCAACCAGGCTTCTCACTT
RG2-3 GCCTGCTTATTGTCTCCTGGTATG
PCR Primer 3
Oligo1 CCA GAC CAG CAGAGG GAC TT
Oligo2 GAA CCT GCG TGC AAT CCA TCT TG
Oligo3 ACC CAG GTA CTC CAT GCC CT


References

Author Jutatip Panaampon, Tatsuya Ogawa, Akira Shiota & Seiji Okada
Title Transgenic expression of human signal regulatory protein alpha (SIRPα) in BALB/c Rag2null/Jak3null immunodeficient mice enhances human lymphoma xenografts by reducing phagocytosis
Journal Transgenic Research
Volume Transgenic Research
Page article number 25 (2026)
Year 2026
PMID


Disease , Applicable field information

Disease name, Applicable field cancer, infectious, Pharmacology






Copyright @ 2021 Kumamoto University. All rights reserved.