| CARD ID | 257 | |
| Type of strain | Transgenic. | |
| Strain name | C57BL/6-Tg(TCR-OT-II.2)Cbn | |
| Internal Code | C57BL/6-TgN(TCR-OT-?)cbn, OT-II, C57BL/6-TgN(TCR-OT-?)Cbn | |
| Submitter | Shigeo Koyasu | |
| Submitter affiliation or code | RIKEN | |
| Stock Type | ||
| Material Transfer Conditions |
Consent to us
|
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| Production method | From other organizations | |
| Origin (In-house) | Organization | |
| Organization code | ||
| Developer | ||
| Origin (From other organizations) | Organization | Walter Eliza Hall Institute |
| Organization code | ||
| Developer | William R. Heath | |
| Year introduced | 2001 / 4 | |
| Introduced Generation | N? | |
| Remarks | ||
| Gene symbol | ? TCRAV2 TCRBV5 ? |
| Gene name | V alpha 2J alpha ? V beta 5D beta ? J beta ? |
| Allele symbol | |
| Allele name | |
| MGI | |
| Chromosome | |
| Gene classification | Gene to express(transgenic) |
| Method | |
| OMIM |
| primerA | PCR primers used to amplify the transgenic TCR α-chain are:, Vα2-5' 5'-AAA GGG AGA AAA AGC TCT CC-3', Cαb 5'-ACA CAG CAG GTT CTG GGT TC-3', which amplify a 160 bp fragment(from Casanova et al., 1991, J.Exp. Med. 174:1371) |
| primerB | As an internal control, primers to insulin can also used. These are:, MINSIXHOF-5' 5'-CGA GCT CGA GCC TGC CTA TCT TTC AGG TC-3', MINSBAMR-5' 5'-CGG GAT CCT AGT TGC AGT AGT TCT CCA G-3', which amplify a 380 bp fragment of the insulin gene. |
| Author | MEGAN J BARNDEN, JAN ALLISON, WILLIAM R HEATH and FRANCIS R CARBONE |
| Title | Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements |
| Journal | Immunology and Cell Biology |
| Volume | 76 |
| Page | 34-40 |
| Year | 1998 |
| PMID | 9553774 |
| Disease name, Applicable field | Immunology |