| CARD ID | 1150 | |
| Type of strain | Targeted mutant. | |
| Strain name | B6;129S2-Pmis2tm1Osb | |
| Internal Code | Spots3-KO | |
| Submitter | Masaru Okabe | |
| Submitter affiliation or code | Genome Information Research (enter Osaka University) | |
| Stock Type | ||
| Material Transfer Conditions |
Others
The RECIPIENT must contact the person listed below in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. mta-egr@biken.osaka-u.ac.jp (Contact to Masahito Ikawa) |
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| Production method | in-house breeding | |
| Origin (In-house) | Organization | |
| Organization code | ||
| Developer | ||
| Origin (From other organizations) | Organization | Research Institute for Microbial Diseases,Osaka University |
| Organization code | Osb | |
| Developer | Ryo Yamaguchi | |
| Year introduced | ||
| Introduced Generation | ||
| Remarks | ||
| Gene symbol | Pmis2 |
| Gene name | Pmis2, sperm specific protein |
| Allele symbol | Pmis2tm1Osb |
| Allele name | Pmis2, sperm specific protein; targeted mutation 1, Masaru Okabe |
| MGI | MGI:1922177, |
| Chromosome | 7 (19.08) , |
| Gene classification | Targeted or trapped gene(knockout etc.) |
| Method | Electroporation |
| OMIM |
| Primer A | GGAATTCGACACCAGGATCTGTCATTG |
| Primer B | AGAATTCTGAAAGAGCAGCTCAGTGGC |
| Primer C | AGGGGAGGAGTAGAAGGTGGCGCGAAGGGG |
| Primer D | AGGATGGATTTCACAGGGCTAAGG |
| Author | Yamaguchi R, Fujihara Y, Ikawa M, Okabe M. |
| Title | Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa |
| Journal | Mol Biol Cell. |
| Volume | 23(14) |
| Page | 2671-9 |
| Year | 2012 |
| PMID |
| Disease name, Applicable field | Development |