CARD R-BASE



Mouse strain


Strain information

CARD ID 2642
Type of strain Targeted mutant.
Strain name C57BL/6-Meikintm1
Internal Code Meikin Ex6 (+/-)
Submitter Ishiguro Kei-ichiro
Submitter affiliation or code Department of Chromosome Biology, Institute of Molecular Embryology and Genetics,Kumamoto University
Stock Type
Material Transfer Conditions Consent to us
Production method in-house breeding
Origin (In-house) Organization Kei-ichiro Ishiguro
Organization code
Developer Institute of Molecular Embryology and Genetics, Kumamoto University
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol Meikin
Gene name meiotic kinetochore factor
Allele symbol Meikintm1
Allele name meiotic kinetochore factor; targeted mutation 1,
MGI MGI:1922097,
Chromosome 11 (32.13) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method
OMIM

PCR Primer 1
Ex3F (common-forward) CCCCAGAGGAAAAGACACCACC
Ex4R (wild-type-reverse) CTCGACAACAAGCTGTCCATCTC
PCR Primer 2
Ex3F (common-forward) CCCCAGAGGAAAAGACACCACC
Neo4R (mutant-reverse) CATGAGTGGGAGGAATGAGCTGGC


References

Author Kim J, Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A., Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S., Tanno Y., Sakuno T., Watanabe Y.
Title Meikin is a conserved regulator of meiosis-I-specific kinetochore function
Journal Nature
Volume 517
Page 466-471
Year 2015
PMID


Disease , Applicable field information

Disease name, Applicable field Molecular biology, Development, Cell biology, Reproduction






Copyright @ 2021 Kumamoto University. All rights reserved.