CARD R-BASE



Mouse strain


Strain information

CARD ID 2161
Type of strain Targeted mutant.
Strain name C57BL/6-Spata4tm1a(KOMP)Osb/3G
Internal Code Spata4 KO
Submitter Ikawa Masahito
Submitter affiliation or code Animal Resource Center for Infectious Diseases Research Institute for Microbial Diseases Osaka University
Stock Type
Material Transfer Conditions Consent to us
Production method in-house breeding
Origin (In-house) Organization Animal Resource Center for Infectious Diseases Research Institute for Microbial Diseases Osaka University
Organization code Osb
Developer Masahito Ikawa
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol Spata4
Gene name spermatogenesis associated 4
Allele symbol Spata4tm1a(KOMP)Osb
Allele name spermatogenesis associated 4, targeted mutation 1a, Animal Resource Center for Infectious Diseases Research Institute for Microbial Diseases Osaka University
MGI MGI:1916531,
Chromosome 8 (29.27) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
#5083 ACTGATGGCGAGCTCAGACC
#5872 TCGGAACTACTAAGCAATCC
PCR Primer 2
#6158 CAGAATTAGAAGTATCCAGGATGATCTCGC
#6159 GGCCAGAATGGAGCTAATGATGAAGAC


References

Author Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM.
Title Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Journal Proc Natl Acad Sci U S A.
Volume 113(28)
Page 7704-10
Year 2016
PMID 27357688


Disease , Applicable field information

Disease name, Applicable field Development






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