CARD R-BASE



Mouse strain


Strain information

CARD ID 1150
Type of strain Targeted mutant.
Strain name B6;129S2-Pmis2tm1Osb
Internal Code Spots3-KO
Submitter Masaru Okabe
Submitter affiliation or code Genome Information Research (enter Osaka University)
Stock Type
Material Transfer Conditions Others
The RECIPIENT must contact the person listed below in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. mta-egr@biken.osaka-u.ac.jp (Contact to Masahito Ikawa)
Production method in-house breeding
Origin (In-house) Organization
Organization code
Developer
Origin (From other organizations) Organization Research Institute for Microbial Diseases,Osaka University
Organization code Osb
Developer Ryo Yamaguchi
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol Pmis2
Gene name Pmis2, sperm specific protein
Allele symbol Pmis2tm1Osb
Allele name Pmis2, sperm specific protein; targeted mutation 1, Masaru Okabe
MGI MGI:1922177,
Chromosome 7 (19.08) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
Primer A GGAATTCGACACCAGGATCTGTCATTG
Primer B AGAATTCTGAAAGAGCAGCTCAGTGGC
Primer C AGGGGAGGAGTAGAAGGTGGCGCGAAGGGG
Primer D AGGATGGATTTCACAGGGCTAAGG


References

Author Yamaguchi R, Fujihara Y, Ikawa M, Okabe M.
Title Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa
Journal Mol Biol Cell.
Volume 23(14)
Page 2671-9
Year 2012
PMID


Disease , Applicable field information

Disease name, Applicable field Development






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