CARD R-BASE



Mouse strain


Strain information

CARD ID 257
Type of strain Transgenic.
Strain name C57BL/6-Tg(TCR-OT-II.2)Cbn
Internal Code C57BL/6-TgN(TCR-OT-?)cbn, OT-II, C57BL/6-TgN(TCR-OT-?)Cbn
Submitter Shigeo Koyasu
Submitter affiliation or code RIKEN
Stock Type
Material Transfer Conditions Consent to us
Production method From other organizations
Origin (In-house) Organization
Organization code
Developer
Origin (From other organizations) Organization Walter Eliza Hall Institute
Organization code
Developer William R. Heath
Year introduced 2001 / 4
Introduced Generation N?
Remarks


Gene information

Gene symbol ? TCRAV2 TCRBV5 ?
Gene name V alpha 2J alpha ? V beta 5D beta ? J beta ?
Allele symbol
Allele name
MGI
Chromosome
Gene classification Gene to express(transgenic)
Method
OMIM

PCR Primer 1
primerA PCR primers used to amplify the transgenic TCR α-chain are:
Vα2-5' 5'-AAA GGG AGA AAA AGC TCT CC-3'
Cαb 5'-ACA CAG CAG GTT CTG GGT TC-3'
which amplify a 160 bp fragment(from Casanova et al., 1991, J.Exp. Med. 174:1371)
primerB As an internal control, primers to insulin can also used. These are:
MINSIXHOF-5' 5'-CGA GCT CGA GCC TGC CTA TCT TTC AGG TC-3'
MINSBAMR-5' 5'-CGG GAT CCT AGT TGC AGT AGT TCT CCA G-3'
which amplify a 380 bp fragment of the insulin gene.


References

Author MEGAN J BARNDEN, JAN ALLISON, WILLIAM R HEATH and FRANCIS R CARBONE
Title Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements
Journal Immunology and Cell Biology
Volume 76
Page 34-40
Year 1998
PMID 9553774


Disease , Applicable field information

Disease name, Applicable field Immunology






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