CARD R-BASE



Mouse strain


Strain information

CARD ID 256
Type of strain Transgenic.
Strain name C57BL/6-Tg(TCR-OT-I)Cbn
Internal Code C57BL/6-TgN(TCR-OT-?)cbn, OT-I, C57BL/6-TgN(TCR-OT-?)Cbn
Submitter Shigeo Koyasu
Submitter affiliation or code RIKEN
Stock Type
Material Transfer Conditions Consent to us
Production method From other organizations
Origin (In-house) Organization
Organization code
Developer
Origin (From other organizations) Organization Walter and Eliza Hall Institute
Organization code
Developer William R. Heath
Year introduced 2001 / 4
Introduced Generation N?
Remarks


Gene information

Gene symbol ? TCRAV2 TCRBV5 ?
Gene name V alpha 2-J alpha 26, V beta 5-D beta 2-J beta 2.6
Allele symbol
Allele name
MGI
Chromosome
Gene classification Gene to express(transgenic)
Method
OMIM

PCR Primer 1
primerA PCR primers used to amplify the transgenic TCR α-chain are:
Vα2-5' 5'-AAA GGG AGA AAA AGC TCT CC-3'
Cαb 5'-ACA CAG CAG GTT CTG GGT TC-3'
which amplify a 160 bp fragment(from Casanova et al., 1991,J.Exp. Med. 174-1371)
primerB As an internal control, primers to insulin can also used. These are:
MINSIXHOF-5' 5'-CGA GCT CGA GCC TGC CTA TCT TTC AGG TC-3'
MINSBAMR-5' 5'-CGG GAT CCT AGT TGC AGT AGT TCT CCA G-3'
which amplify a 380 bp fragment of the insulin gene.


References

Author Kristin A. Hogquist, Stephen C. Jameson, William R. Heath, Jane L. Howard, Michael J. Bevan, and Francis R. Carbone
Title T Cell Receptor Antagonist Peptides Induce Positive Selection
Journal Cell
Volume 76
Page 17-27
Year 1994
PMID 8287475


Disease , Applicable field information

Disease name, Applicable field Immunology






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