CARD R-BASE



Mouse strain


Strain information

CARD ID 1194
Type of strain Targeted mutant.
Strain name B6;CB-Ttrtm2(hTTRV30M)Imeg
Internal Code TtrhV30M/hV30M
Submitter Ken-ichi YAMAMURA
Submitter affiliation or code Center for Animal Resources and Development Kumamoto University
Stock Type
Material Transfer Conditions Consent to us
All embryos were collected by mating between male hemizygous C57BL/6-Tg(6.0hTTRMet30)15Imeg mice and wild type female mice. Thus, genotypes of these embryos are either hemizygous or wild type. Similary, embryos were collected by mating between heterozygous B6:CB-Ttr and wild type mice. Thus, embryos are heteorzygous. For genotyping for C57BL/6-Tg(6.0hTTRMet30) mouse, please see the manuscript by Kohno et al 1997. For B6:CB-Ttr, please see the manuscript by Zhao et al. 2008 and hTTR KI genotyping. ID 1194 is a line in which a human mutant TTR (V30M) cDNA is inserted into the mouse Ttr locus. Please see the manuscript by Zhao et al. 2008.
Production method in-house breeding
Origin (In-house) Organization Division of Developmental Genetics, IMEG
Organization code Imeg
Developer Li Zhenghua
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol Ttr
Gene name transthyretin
Allele symbol Ttrtm2(hTTRV30M)Imeg
Allele name
MGI MGI:98865,
Chromosome 18 (7) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
A9-s 5'-CGT AGA GCG AGT GTT CCG-3'
sp-A 5'-CAG TGT ATA TCA TTG TAA CC-3'


Disease , Applicable field information

Disease name, Applicable field Metabolism, Neurobiology, Dermatology, Anatomy


EGTC

EGTC番号




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