CARD ID | 256 | |
Type of strain | Transgenic. | |
Strain name | C57BL/6-Tg(TCR-OT-I)Cbn | |
Internal Code | C57BL/6-TgN(TCR-OT-?)cbn, OT-I, C57BL/6-TgN(TCR-OT-?)Cbn | |
Submitter | Shigeo Koyasu | |
Submitter affiliation or code | RIKEN | |
Stock Type | ||
Material Transfer Conditions |
Consent to us
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Production method | From other organizations | |
Origin (In-house) | Organization | |
Organization code | ||
Developer | ||
Origin (From other organizations) | Organization | Walter and Eliza Hall Institute |
Organization code | ||
Developer | William R. Heath | |
Year introduced | 2001 / 4 | |
Introduced Generation | N? | |
Remarks |
Gene symbol | ? TCRAV2 TCRBV5 ? |
Gene name | V alpha 2-J alpha 26, V beta 5-D beta 2-J beta 2.6 |
Allele symbol | |
Allele name | |
MGI | |
Chromosome | |
Gene classification | Gene to express(transgenic) |
Method | |
OMIM |
primerA | PCR primers used to amplify the transgenic TCR α-chain are:, Vα2-5' 5'-AAA GGG AGA AAA AGC TCT CC-3', Cαb 5'-ACA CAG CAG GTT CTG GGT TC-3', which amplify a 160 bp fragment(from Casanova et al., 1991,J.Exp. Med. 174-1371) |
primerB | As an internal control, primers to insulin can also used. These are:, MINSIXHOF-5' 5'-CGA GCT CGA GCC TGC CTA TCT TTC AGG TC-3', MINSBAMR-5' 5'-CGG GAT CCT AGT TGC AGT AGT TCT CCA G-3', which amplify a 380 bp fragment of the insulin gene. |
Author | Kristin A. Hogquist, Stephen C. Jameson, William R. Heath, Jane L. Howard, Michael J. Bevan, and Francis R. Carbone |
Title | T Cell Receptor Antagonist Peptides Induce Positive Selection |
Journal | Cell |
Volume | 76 |
Page | 17-27 |
Year | 1994 |
PMID | 8287475 |
Disease name, Applicable field | Immunology |