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CARD Mouse Bank


Mouse strain


Strain information

CARD ID 2647
Type of strain Targeted mutant.
Strain name C57BL/6-Stra8em2
Internal Code Stra8KO-3FH Ex9 (Lx20-G1bp del AG)
Submitter Ishiguro Kei-ichiro
Submitter affiliation or code Department of Chromosome Biology, Institute of Molecular Embryology and Genetics,Kumamoto University
Stock Type
Material Transfer Conditions Consent to us
Production method in-house breeding
Origin (In-house) Organization Institute of Molecular Embryology and Genetics, Kumamoto University
Organization code
Developer Kei-ichiro Ishiguro
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks This allele was generated by ssODN methods, by injecting Crisp-Cas9 into fertilized egg of Stra8-3xFLAG-HA-2A-GFP genetic background.


Gene information

Gene symbol Stra8
Gene name stimulated by retinoic acid gene 8
Allele symbol Stra8em2
Allele name stimulated by retinoic acid gene 8; endonuclease-mediated mutation 2,
MGI MGI:107917,
Chromosome 6 (15.2) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
St8-24996F AGGCCCAGCATATGTCTAACATCAG
KI92ES-29564R AGAAGGCTTTTGGAAGCAGCCTTTC


References

Author Ishiguro K, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M, Sugimoto M, Fujimura S, Hosokawa M, Chuma S, Ko S.H.M, Araki K, Niwa H.
Title MEIOSIN directs the switch from mitosis to meiosis in mammalian germ cells.
Journal Dev. Cell
Volume 52(4)
Page 429-445
Year 2020
PMID


Disease , Applicable field information

Disease name, Applicable field Molecular biology, Development






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