CARD R-BASE



Mouse strain


Strain information

CARD ID 2104
Type of strain Transgenic., Targeted mutant.
Strain name B6;129-Pccatm1ToruTg(SAP-Pcca)47Toru
Internal Code PCCA KO/ PCCA TG
Submitter Miyazaki Toru
Submitter affiliation or code Lab of Molecular Biomedicine for Pathogenesis, Center for Disease Biology and Integrative Medicine (CDBIM), The University of Tokyo
Stock Type
Material Transfer Conditions Consent to us
Production method in-house breeding
Origin (In-house) Organization
Organization code
Developer
Origin (From other organizations) Organization
Organization code
Developer
Year introduced
Introduced Generation
Remarks


Gene information

Gene symbol Pcca
Gene name propionyl-Coenzyme A carboxylase, alpha polypeptide
Allele symbol tg(SAP-Pcca)
Allele name
MGI MGI:97499,
Chromosome
Gene classification Gene to express(transgenic)
Method MicroInjection
OMIM
Gene symbol Pcca
Gene name propionyl-Coenzyme A carboxylase, alpha polypeptide
Allele symbol Pccatm1Timy
Allele name propionyl-Coenzyme A carboxylase, alpha polypeptide, targeted mutation 1, Toru Miyazaki
MGI MGI:97499,
Chromosome 14 (65.99) (14E5) ,
Gene classification Targeted or trapped gene(knockout etc.)
Method Electroporation
OMIM

PCR Primer 1
beta-globin 5 TGC TGG TTA TTG TGC TGT CTC ATC
PCCA 300R AAT GGC AAC TGT CTT GAT GCC CAT
PCR Primer 2
RV13 GTA TCC ATC ATG GCT GAT GCA A
RV14 AAC GCT ATG TCC TGA TAG CGG T


References

Author Miyazaki T, Ohura T, Kobayashi M, Shigematsu Y, Yamaguchi S, Suzuki Y, Hata I, Aoki Y, Yang X, Minjares C, Haruta I, Uto H, Ito Y, Müller U
Title Fatal propionic acidemia in mice lacking propionyl-CoA carboxylase and its rescue by postnatal, liver-specific supplementation via a transgene.
Journal J Biol Chem
Volume 276
Page 35995-9
Year 2001
PMID


Disease , Applicable field information

Disease name, Applicable field Metabolism






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